Hamdan Medical Journal (previously the Journal of Medical Sciences)

Table of Contents  

Tissue Specific Stem Cells - The Lesson from Hematopoietic Stem Cell Transplantation

Donald Metcalf
Published in : Journal of Medical Sciences ; Vol 1, No 3 (2008)
DOI : 10.2174/1996327000801030084


In the clinical application of tissue-specific stem cells, the lessons from hematopoietic stem cell transplantation are first, the importance of selective clonal culture assays to characterize and monitor stem cells, and second, the identification, mass production and use of tissue-specific growth regulatory factors to promote the growth of transplanted stem cells. This review will discuss some of the questions at issue in the currently popular subject of stem cells. In particular, the discussion will try to set the questions in the context of likely clinical applications. Are such applications really feasible and what information is still missing that is likely to be needed for successful clinical applications?  The review will use hematopoietic stem cells as a useful case study where, at least some of the lessons emerging are likely to be relevant for other tissue-specific stem cells.

View article in  :   PDF    


Ratajczak MZ, Machalinski B, Wojakowski W,Ratajczak J, Kucia M. A hypothesis for anembryonic origin of pluripotent ct-4(+) stem cells in adult bone marrow and other tissues.eukemia 2007; 21: 860-7.

Virchow R. Editorial Archive fuerpathologische. Anatomie und Physiologie fuerKlinische Medizin 1855; 8: 23-54.

Blau HM, Brazelton TR, Weimann JM. The evolving concept of a stem cell: entity or function? Cell 2001; 105: 829-41.

Gage FH. Mammalian neural stem cells.Science 2000; 287: 1433-8.

Metcalf D. Hematopoietic stem cells and tissuestem cells: Current concepts and unanswered questions. Stem Cells 2007; 25:2390-5.

Boyer LA, Mathur D, Jaenisch R. Molecular control of pluripotency. Curr Opin Genet Dev 2006; 16: 455-62.

Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126: 663-76.

Maherali N, Sridharan R, Xie W, et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 2007; 1: 55-70.

Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stemcells. Nature 2007; 448: 313-7.

Wernig M, Meissner A, Foreman R, et al. In vitroreprogramming of fibroblasts into a pluripotent ES cell-like state.Nature 2007; 448: 318-24.

Morrison SJ. Stem cell potential: can anything make anything? Curr Biol 2001; 11: R7-9.

Bjornson CR, Rietze RL, Reynolds BA, Magli MC,Vescovi AL. Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo. Science 1999; 283: 534-37.

Metcalf D. On hematopoietic stem cell fate.Immunity 2007a; 26: 669-73.

Krause DS, Theise ND, Collector MI, et al. Multiorgan,multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 2001; 105:369-77.

Till JE, McCulloch EA. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. RadiatRes 1961; 14: 213-2.

Alexander WS, Roberts AW, Nicola NA, Li R,Metcalf D. Deficiencies in progenitor cells of multiple hematopoietic lineages and defective megakaryocytopoiesis in mice lacking the thrombopoietic receptor c-Mpl.Blood 1996; 87: 2162-70.

Metcalf D, Moore, MAS. Hematopoietic Cells.North-Holland, Amsterdam, 1971.

Moore MA, Metcalf D. Ontogeny of the haemopoietic system: yolk sac origin of in vivo and in vitro colony forming cells in the developing mouse embryo. Br J Haematol 1970; 18: 279-96.

Godin I, Dieterlen-Lievre F, Cumano A.Emergence of multipotent hemopoietic cells in the yolk sac and paraaortic hnopleura in mouse embryos, beginning at 8.5 days postcoitus. Proc Natl Acad Sci USA 1995; 92:773-7.

Bradley TR, Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci 1966; 44: 287-99.

Pluznik DH, Sachs L. The induction of clones of normal mast cells by a substance from conditioned medium. Exp Cell Res 1966; 43:553-63.

Metcalf D, Nicola NA. The hemopoietic colony-stimulating factors (Cambridge, UK,Cambridge University Press), 1995.

Akashi K, Traver D, Miyamoto T, Weissman IL. A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Nature 2000;404: 193-7.

Kondo M, Weissman IL, Akashi, K. Identification of clonogenic common lymphoid progenitors in mouse bone marrow. Cell 997; 91: 661-72.

Iwasaki H, Mizuno S, Wells RA, Cantor AB,Watanabe S, Akashi K. GATA-1 converts lymphoid and myelomonocytic progenitors to the megakaryocyte/erythrocyte lineages.Immunity 2003; 19: 451-62.

Attar EC, Scadden DT. Regulation of hematopoietic stem cell growth. Leukemia 2004; 18; 1760-8.

Duhrsen U, Villeval J-L, Boyd J, Kannourakis G,Morstyn G, Metcalf D. Effects of recombinant human granulocyte-colony stimulating factor on hemopoietic progenitor cells in cancer patients. Blood 1988; 72: 2074-81.

Add comment 

Home  Editorial Board  Search  Current Issue  Archive Issues  Announcements  Aims & Scope  About the Journal  How to Submit  Contact Us
Find out how to become a part of the HMJ  |   CLICK HERE >>
© Copyright 2012 - 2013 HMJ - HAMDAN Medical Journal. All Rights Reserved         Website Developed By Cedar Solutions INDIA