Hamdan Medical Journal (previously the Journal of Medical Sciences)

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Targeting Endothelial Cell Surface Receptors: Novel Mechanisms of Microvascular Endothelial Barrier Transport

Asrar B Malik
Published in : Journal of Medical Sciences ; Vol 2, No 1 (2009)
DOI : 10.2174/1996327000902010013

Abstract


The delivery of novel biological therapeutic agents across the vascular endothelial barrier (such as monoclonal antibodies targeting specific receptors; e.g., anti-epithelial derived growth factor receptor antibody) represents a major challenge in drug delivery. The situation becomes even more complex in protein delivery across the much more restrictive bloodbrain barrier. In studying endothelial barrier function, we have identified new mechanisms of delivery of biologic therapeutics. Maximum anti-tumor effects or other protective effects of the specific protein thus can be achieved at doses below the maximum tolerated dose. In this review, I summarize below some of key recent development in this area with emphasis on our work.


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References


Tiruppathi C, Naqvi T, Wu Y, Vogel SM, Minshall RD, Malik AB. Albumin mediates the transcytosis of myeloperoxdase by means of caveolae in endothelial cells. Proc Natl Acad Sci USA 2004; 101: 7699-704.

Schnitzer JE. Caveolae: from basic trafficking mechanism to targeting transcytosis for tissuespecific drug and gene delivery in vivo. Adv Drug Deliv Rev 2001; 49: 265-80.

McIntosh DP, Tan XY, Oh P, Schnitzer JE. Targeting endothelium and its dynamic caveolae for tissue-specific transcytosis in vivo: A pathway to overcome cell barriers to drug and gene delivery. Proc Natl Acad Sci USA 2002; 99: 1996-2001.

Tiruppathi C, Finnegan A, Malik AB. Isolation and characterization of a cell surface albumin binding protein from vascular endothelial cells. Proc Natl Acad Sci USA 1996; 93: 250-4.

Tiruppathi C, Song W, Bergenfeldt M, Sass P, Malik AB. Gp60 activation mediates albumin transcytosis in endothelial cells by tyrosine kinase-dependent pathway. J Biol Chem 1997; 272: 25968-275.

Minshall RD, Tiruppathi C, Vogel SM, et al. Endothelial cell surface gp60 activates vesicle formation via Gi-coupled Src kinase signaling Pathway. J Cell Biol 2000; 150: 1057-69.

Vogel SM, Minshall RD, Pilipovic M, Tiruppathi C, Malik AB. Albumin uptake and transcytosis in endothelial cells in vivo induced by albuminbinding protein (gp60). Am J Physiol 2001; 281: L958-68.

Shajahan AN, Timblin B, Sandoval R, Tiruppathi C, Malik AB, Minshall RD. Role of Src-Induced Dynamin-2 Phosphorylation in Caveolae- Mediated Endocytosis in Endothelial Cells. J Biol Chem 2004; 279: 20392-400.

John TA, Vogel SM, Minshall RD, et al. Evidence for the role of alveolar epithelial gp60 in active transalveolar albumin transport in the rat lung. J Physiol 2001; 533: 547-59.

Vogel SM, Easington CR, Minshall RD, et al. Evidence of transcellular permeability pathway in microvessels. Microvasc Res 2001; 61: 87-101.

Schnitzer JE, Oh P. Albondin-mediated capillary permeability to albumin. J Biol Chem 1994; 269: 6072-82.

Schnitzer JE, Oh P. Antibodies to SPARC inhibit albumin binding to SPARC, gp60, and microvascular endothelium. Am J Physiol 1992; 263: H 1872-9.

Niles WD, Malik AB. Endocytosis and exocytosis events regulate vesicle traffic in endothelial cells. J Membr Biol 1999; 167: 85-101.

Mehta D, Malik AB. Signaling mechanisms regulating endothelial permeability. Physiol Rev 2006; 86: 279-367.





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