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Effect of combined administration of nicotine and caffeine on the prostate of adult rats

BM Bashykh, NA Rajeh
Published in : HAMDAN MEDICAL JOURNAL ; Vol 8, No 4 (2015): Supplement Issue
DOI : 10.7707/hmj.522


Introduction: Nicotine and caffeine have been shown to be reproductive toxicants in animals and are associated with a high risk of cancer in several in vitro  studies.

Objectives: To investigate the combined toxic effect of nicotine and caffeine on rat prostate histology and serum testosterone level.

Materials and methods: Wistar rats (250– 300g) were injected with 100mg/kg body weight of caffeine by intraperitoneal injection daily for 1 month. Concomitantly, nicotine was injected at 10mg/kg body weight three times per week subcutaneously. After 1 month of injections, serum was taken from the retro-orbital sinus of all animals then rats were sacrificed by cervical dislocation. All prostates were removed for further histological preparation and examination.

Results: Haematoxylin and eosin staining showed that nicotine at the tested dose increased interacinar spaces with reductions in stromal tissue (loose stroma), and many congested blood vessels were noted in the stroma. The acini themselves became dilated and thin walled with poorly infolded mucosa with reductions in the height of epithelial lining and flattening of columnar cells. An increase in testosterone level (P <0.05) was noted in the group treated with caffeine alone and in the group treated with both drugs.

Conclusions: At the used dose, nicotine produced toxic effects in the prostate of male rats that can be antagonized by concomitant treatment with caffeine.

Acknowledgements: Dr Nisreen Rajeh for supervising this work.


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